Titlebook: RNA Isolation and Characterization Protocols; Ralph Rapley,David L. Manning Book 1998 Springer Science+Business Media New York 1998

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Isolation of Messenger RNA,mRNA comprises approx l–5% of total cellular RNA. Although the actual amount depends on the type of cell and its physiological state, at any given time approx 12,000 genes are being transcribed with approx 500,000 mRNA molecules present in each mammalian cell.

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Isolating RNA with the Cationic Surfactant, Catrimox-14,phase, and the cationic head groups bind to the nucleic acid electrostatically. The precipitate can be dissolved in organic solvents, or it can be redissolved in water by the addition of salt. Selected cationic surfactants are capable of lysing cells, by solubilizmg their protem and lipid components.

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RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues,many purposes, a simple qualitative analysis of the presence of a specific RNA in tissues may be sufficient, for instance, to establish the persistence in tissues of the genome of an RNA virus. However, when gene expression levels are needed, quantiation of the specific mRNA is an important task.

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Wolfgang Uckert,Wolfgang Walther,Ulrike Steine- rienced scientists from universities, research laboratories and industry have the opportunity to discuss their most recent results, and where students and young scientists can learn about the present status of research and applications from some of the most eminent workers in their field. In comm

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Christopher E. Dahle,Donald E. Macfarlanee- rienced scientists from universities, research laboratories and industry have the opportunity to discuss their most recent results, and where students and young scientists can learn about the present status of research and applications from some of the most eminent workers in their field. In comm

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Giorgio Stanta,Serena Bonin,Rosella Perinrtion. This phase is related to the choice of the origin of coordinates and then to the translational symmetry of the system. Hence this sliding motion, or sliding conductivity, is sensitive to the impurity scattering and the Umklapp scattering [8]. These scattering mechanisms result in impurity and

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