Titlebook: RNA Interference and Cancer Therapy; Methods and Protocol Lekha Dinesh Kumar Book 2019 Springer Science+Business Media, LLC, part of Spring

Basal-Ganglia

Relative Quantification of siRNA Strand Loading into Ago2 for Design of Highly Active siRNAs,ivity and specificity, selection of the appropriate siRNA strand for loading into Ago2 is essential. Here, we provide a protocol to quantify the relative loading of individual siRNA strands into Ago2, one factor in determining the capacity of a siRNA to achieve silencing activity and target specificity.

THROB

Minicell-Based Targeted Delivery of shRNA to Cancer Cells: An Experimental Protocol,ized by fluorescence microscopy and transmission electron microscopy. In the next step, targeting ligand was conjugated on the minicell surface for the active targeting of cancer cell surface receptors. Eventually, target-specific delivery of minicells was explored in vitro in selected cancer cell line and in vivo in mice bearing tumor xenograft.

Asparagus

Design of Eco-Friendly Gold Nanoparticles for Cancer Treatment,highly biocompatible GNP using a natural gum, i.e., xanthan gum (XG). Moreover, due to the presence of mannose moiety in XG, these XG-stabilized GNP may also act as self-targeted drug carriers for the delivery of chemotherapeutic agents/siRNA/shRNA to mannose receptor overexpressing cancer cells.

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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820171.jpg

Visual-Acuity

https://doi.org/10.1007/978-1-4939-9220-1Cancer treatment; Therapeutic tools; RNAi-based strategies; Bio-drugs; Drug development; Targeted deliver

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incarcerate

Pelvic-Floor

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