Titlebook: RNA Editing; Methods and Protocol Ernesto Picardi,Graziano Pesole Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2

Corral

Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820160.jpg

和平主義

https://doi.org/10.1007/978-1-0716-0787-9plant organelles; metazoan; DNA-RNA changes; APOBEC; mouse tissues; interferonopathies

起草

Ernesto Picardi,Graziano PesoleIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

揉雜

Quantitative Analysis of Adenosine-to-Inosine RNA Editing, analysis of electropherogram peak heights. Here, we briefly review and compare these methods of RNA editing quantification, as well as provide experimental protocols by which such analyses may be achieved.

Living-Will

RNA Editing in Human and Mouse Tissues,s simultaneously. Our experimental workflow includes the harvesting of high-quality RNA samples and the construction of different high-throughput sequencing libraries. We also delineate the computational steps needed to analyze the sequencing data from an Illumina platform.

COMA

MicroRNA Editing Detection and Function: A Combined In Silico and Experimental Approach for the Ideediting machinery also?involves important miRNAs (either oncomiRs or tumor-suppressor miRNAs). Herein we describe a combined in silico and experimental approach for the detection of edited miRNAs and the identification and validation of their target genes potentially involved in cancer progression or invasion.

altruism

motor-unit

Aerophagia

Computational Detection of Plant RNA Editing Events,eq data processing, RNA read alignment, and RNA editing site detection. Each step is discussed in sufficient detail to be implemented by the reader. As a study case, the framework will be used with publicly available sequencing?data to detect C-to-U RNA editing sites in the?coding sequences of the mitochondrial genome of ..

正常

Adenosine-to-Inosine RNA Editing Enzyme ADAR and microRNAs,on that can introduce mutations into the precursor of microRNA (miRNA). It can also regulate miRNA processing independently of A-to-I RNA editing. This chapter outlines the role of an A-to-I RNA editing enzyme ADAR in miRNA processing and the experimental systems used to analyze the interaction between miRNAs and ADAR.