Titlebook: RNA Bioinformatics; Ernesto Picardi Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2021 Computation

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for Isoform Quantification in Large-Scale RNA Sequencing,ysis, there is growing evidence that most mammalian genes are alternatively spliced to generate different isoforms that can be subsequently translated to protein molecules with diverse or even opposing biological functions. Quantifying the expression levels of these isoforms is key to understanding

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Differential Expression Analysis of Long Noncoding RNAs,xpression genes are commonly observed in lncRNA and need to be effectively accommodated in differential expression analysis. In this chapter, we describe a protocol based on existing R packages for lncRNA differential expression analysis, including lncDIFF, ShrinkBayes, DESeq2, edgeR, and zinbwave,

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RNA Editing Detection in HPC Infrastructures,fects and its deregulation has been linked to a variety of human disorders including neurological and neurodegenerative diseases and cancer. Despite its biological relevance, the detection of RNA editing variants in large transcriptome sequencing experiments (RNAseq) is yet a challenging computation

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