Titlebook: Quorum Sensing; Methods and Protocol Livia Leoni,Giordano Rampioni Book 2018 Springer Science+Business Media LLC 2018 Bacteria.Bacterial so

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Imaging ,-Acyl Homoserine Lactone Quorum Sensing In Vivor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a . strain together with the . monitor strain can be investigated at different time points postinfection. Epifluoresc

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Assessing , Autoinducer Effects on Mammalian Epithelial Cellserium . may use quorum sensing to communicate with other microorganisms and mammalian cells to alter gene expression. Here, we present methodologies to evaluate the effects of .-(3-oxo-dodecanoyl)-.-homoserine lactone (3O-C.-HSL) on Caco-2 cell monolayers. First, we describe a method for assessing b

Mechanics

Detection of 2-Alkyl-4-Quinolones Using BiosensorsQ biosensor. Light output by the bioreporter correlates with the AQ content of the sample. The assays described are simple to perform, do not require sophisticated instrumentation, and are highly amenable to screening large numbers of bacterial samples.

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Fluorescence Quenching Studies of γ-Butyrolactone-Binding Protein (CprB) from , A3(2)ing methods and fluorescence lifetime decay kinetics using CprB protein from . A3(2) as a model system. Interaction data between CprB and two chemically synthesized GBLs involved in quorum sensing, Cp1 and Cp2, have been used as example.

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Assessing , Autoinducer Effects on Mammalian Epithelial Cellsthelial junction proteins. Lastly, we will detail imaging techniques to visualize Caco-2 barrier disruption following exposure to 3O-C.-HSL through the use of confocal laser scanning microscopy (CLSM) and a super resolution technique, stimulated emission depletion (STED) microscopy, to achieve nanoscale visualization of Caco-2 monolayers.