Titlebook: Quantum Dots; Applications in Biol Adriana Fontes,Beate S. Santos Book 2020Latest edition Springer Science+Business Media, LLC, part of Spr

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Measuring the Hydrodynamic Radius of Colloidal Quantum Dots by Fluorescence Correlation Spectroscopye-based techniques, for over two decades. A great variety of QD syntheses and conjugations are available, and tailoring these for the desired application requires a refined structural characterization. Life science applications rely on the interaction of QDs with biostructures; hence, the knowledge

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Encapsulating Quantum Dots in Lipid–PEG Micelles and Subsequent Copper-Free Click Chemistry Bioconjuapolar organic solvents to water, the optical properties of the fluorescent nanoparticles must be maintained; additionally, the resulting colloid should be monodisperse and stable against aggregation. Furthermore, the hydrophilic coating should confer functional groups or conjugation handles to the

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Phase Transfer and DNA Functionalization of Quantum Dots Using an Easy-to-Prepare, Low-Cost Zwitteriaining these characteristics can be highly specialized or expensive. We describe a straightforward, low-cost protocol for functionalizing poly(isobutylene-.-maleic anhydride) (PIMA) with moieties that anchor to the QD surface (histamine), impart hydrophilicity [(2-aminoethyl)trimethylammonium chlori

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Dextran-Functionalized Quantum Dot Immunoconjugates for Cellular Imagingerties and surface area that supports multivalent conjugation of biomolecules. An important design consideration for effective use of these materials is a hydrophilic, biocompatible surface chemistry that provides colloidal stability and minimizes nonspecific interactions with biological molecules a

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Labeling Neuronal Proteins with Quantum Dots for Single-Molecule Imaginges in the brain. Here, we describe how nanometer-sized fluorescent semiconductors called quantum dots (QD) can be used to label neuronal proteins in a single QD imaging format. We detail two generalizable protocols accompanied by experimental considerations giving the user options in approach tailor

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Quantitative Chemical Delivery of Quantum Dots into the Cytosol of Cellsn attractive solution to this problem due to their exceptional photostability and brightness. Here, we describe in detail a protocol to chemically deliver functionalized QDs into the cytosol of living cells, based on cell-penetrating poly(disulfide)s (CPDs). This protocol is highly efficient and del

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Resazurin-Based Assay to Evaluate Cell Viability After Quantum Dot Interactiones in cell viability using a variety of methods, especially colorimetric ones. One of the most applied tests is the MTT assay. In comparison to MTT, for example, the resazurin-based method has the main advantage of not evaluating the cells directly, thus eliminating false-positive results that may a

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