Titlebook: Quantitative Real-Time PCR; Methods and Protocol Roberto Biassoni,Alessandro Raso Book 2014 Springer Science+Business Media New York 2014 D

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Methods in Molecular Biologyhttp://image.papertrans.cn/q/image/780966.jpg

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Circulating Cell-Free DNA in Cancer,This papers deals with the preanalytical and analytical phase of cell-free DNA analysis, highlighting some criticism on sample collection and extraction. We describe a method to accurately quantify total cfDNA in plasma and our particular approach to the measurement of tumor deriving cfDNA.

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Introduction to Digital PCR,a routine molecular biology assay. This exciting new technique provides quantitative and detection capabilities that by far surpass other methods currently used. This chapter is an overview of some of the applications currently being performed using dPCR as well as the fundamental concepts and techniques this technology is based on.

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