Titlebook: Quantitative PCR Protocols; Bernd Kochanowski,Udo Reischl Book 1999 Humana Press 1999

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Comparison of Competitive PCR and Positive Control-Based PCR an understanding of the critical parameters involved in quantitative amplification. Accepted parameters can be summarized as a series of choices: external vs internal standard, exogenous vs endogenous standard, competitive vs noncompetitive amplification, and exponential vs plateau amplification (.–.).

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Book 1999brilliant technique for in vitro amplification of nucleic acids. Although PCR has had a profound impact in many areas of research, contrary to exp- tation its routine application to the quantitation of nucleic acids has proven problematic in several aspects. The shortcomings are principally caused b

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Quantitative RT-PCRDNA from the RNA template and PCR. In practice, the exponential nature of PCR and the practical aspects of performing PCR pose the most serious obstacles to obtaining quantitative information. With some adaptations, however, RT-PCR can yield accurate quantitative results.

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Competitor Calibration and Analysis of Competitive Amplified PCR Products by High-Performance Liquidget sample and therefore compete for the common primers and reagents in the same reaction tube. After coamplification, both products are distinguished by characteristic features, e.g., size, mostly by electrophoretic methods, or by probe-specific hybridization, e.g., PCR-ELISA (.,.).