| 書目名稱 | Protein Lipidation | | 副標(biāo)題 | Methods and Protocol | | 編輯 | Maurine E. Linder | | 視頻video | http://file.papertrans.cn/762/761700/761700.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | .This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins. The book is organized into seven parts: Part One describes chemically-based strategies to identify substrates for protein lipidation that can be applied to individual proteins or globally using proteomics. Part Two focuses on the enzymes that remove fatty acids from proteins and provides methods to monitor protein biogenesis and palmitate turnover. Part Three addresses biochemical and cellular characterization of DHHC S-acyltransferases, a family of enzymes with 23 members encoded by the human genome. Part Four presents the SwissPalm 2 database and tips on how to use it effectively. Part Five focuses on fatty acylation that occurs in the lumen of the secretory pathway. Parts Six and Seven conclude the book with methods to produce and assay lipid-modified and integral membrane proteins. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readi | | 出版日期 | Book 2019 | | 關(guān)鍵詞 | fatty acylation; lipid modifications; cysteine modifications; Mass Spectrometry; enzyme | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-9532-5 | | isbn_softcover | 978-1-4939-9534-9 | | isbn_ebook | 978-1-4939-9532-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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