| 書目名稱 | Protein Chromatography | | 副標題 | Methods and Protocol | | 編輯 | Dermot Walls,Sinéad T. Loughran | | 視頻video | http://file.papertrans.cn/762/761645/761645.mp4 | | 概述 | Features contributions from experienced scientists with hands-on expertise in the field presenting step-by-step methodologies, tips, and associated explanatory notes.Includes a large section on the ge | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources.In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike.The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography.Written in the highly successful Methods in Molecular Biology? series format, protocols chapters include introductions to their respective subjects, lists of the n | | 出版日期 | Book 2011 | | 關(guān)鍵詞 | Chromatographic methods; Microfluidics; Native protein isolation; Protein biochemistry; Protein quantita | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-60761-913-0 | | isbn_softcover | 978-1-4939-6129-0 | | isbn_ebook | 978-1-60761-913-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2011 |
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