| 書目名稱 | Protein Chromatography | | 副標(biāo)題 | Methods and Protocol | | 編輯 | Dermot Walls,Sinéad T. Loughran | | 視頻video | http://file.papertrans.cn/762/761643/761643.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | .This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Protein Chromatograp | | 出版日期 | Book 2017Latest edition | | 關(guān)鍵詞 | protein stability and storage; avoiding proteolysis during chromatography; protein quantitation; immuno | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-4939-6412-3 | | isbn_softcover | 978-1-4939-8191-5 | | isbn_ebook | 978-1-4939-6412-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2017 |
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