| 書目名稱 | Peptide Research Protocols | | 副標(biāo)題 | Endothelin | | 編輯 | Janet J. Maguire,Anthony P. Davenport | | 視頻video | http://file.papertrans.cn/744/743524/743524.mp4 | | 概述 | Includes supplementary material: | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | Tantalizing new research frontiers are opening in peptide research, where the endothelins constitute an important class of signals that can be manipulated therapeutically to treat cardiovascular disease. In Peptide Research Protocols: Endothelin, a panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of peptide research by focusing on the endothelin system-from its synthesis and metabolism, to its function in health and disease. These methods use state-of-the-art molecular techniques to quantify the expression of mRNA, here using both endothelin receptors and the endothelin-converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies, and how radioligand binding techniques can provide additional information on receptor characterization. In vitro assays are presented to determine the function of peptides in isolated preparations, to characterize new receptor ligands, and to provide information on the tissue-specific processing of precursor peptides-all in the endothelin system. S | | 出版日期 | Book 2002 | | 版次 | 1 | | doi | https://doi.org/10.1385/1592592899 | | isbn_softcover | 978-1-61737-291-9 | | isbn_ebook | 978-1-59259-289-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2002 |
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