| 書目名稱 | PCR Protocols | | 編輯 | John M. S. Bartlett,David Stirling | | 視頻video | http://file.papertrans.cn/741/740071/740071.mp4 | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. | | 出版日期 | Book 20032nd edition | | 關(guān)鍵詞 | DNA; Microarray; PCR; Polymerase Chain Reaction; Quantitative PCR; SNP; Single Nucleotide Polymorphism; Vir | | 版次 | 2 | | doi | https://doi.org/10.1385/1592593844 | | isbn_ebook | 978-1-59259-384-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2003 |
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