Titlebook: Oxidants and Antioxidants; Ultrastructure and M Donald Armstrong Book 2002 Humana Press 2002

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Cytochemical Localization of H2O2 in Biological Tissues The major problem in determining the role of reactive oxygen species (ROS) has been that these short-lived species are difficult to measure in vivo (.). ROS in cells and tissues have been demonstrated by a number of methods. Effects of free radical scavengers such as superoxide dismutase (SOD), cat

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4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibodyde. These ROS can peroxidize membrane lipids of a cell and its organelles, and can also attack DNA or protein (.). During the process of lipid peroxidation, polyunsaturated fatty acids (PUFA), especially linoliec acid, arachidonic acid, and docosahexaenoic acid, in biomembranes are degraded to a gre

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Indirect Immunofluorescence Detection of Protein-Bound 4-Hydroxynonenal in Tissue Sections and Isolaing the process of lipid peroxidation (.) (.). The latter is an autocatalytic, self-propagating sequence of free radical reactions, ultimately resulting in the fragmentation of the carbon atom chains of unsaturated fatty acids esterified in phospholipids of cellular membranes, which can be set into

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Methods for Studying the Binding of Advanced Glycated Proteins to Receptors for Advanced Glycation Etides, and phospholipids. They are formed in physiological systems by glycation reactions of glucose and physiological α-oxoaldehydes, particularly glyoxal, methylglyoxal, and 3-deoxyglucosone (3-DG) with amino and guanidino groups. The concentration of intracellular AGEs is kept at alow level by pr

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