Titlebook: Organ Regeneration; 3D Stem Cell Culture Takashi Tsuji Book 2017 Springer Science+Business Media LLC 2017 Cerebellar.Stem Cell.Transplantat

起來(lái)了

Generation of a Bioengineered Lacrimal Gland by Using the Organ Germ Method,blem leading reduction of visual function. Here, we describe protocols for lacrimal gland germ regeneration using the organ germ method and methods for analyzing the function of the bioengineered lacrimal gland after its transplantation in vivo.

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Liver Regeneration Using Cultured Liver Bud, masses. We named these iPSC liver buds (iPSC-LBs) because they resemble the developing liver bud from the perspective of gene expression, cell proliferation, and cell proportion. This liver bud culture system provides a novel approach for future clinical applications, for drug development, and as a tool for studying human development.

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Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture,y to self-organize stratified neural tissue including 3D-retina. Furthermore, we have reported that ESC-derived retinal tissue can form an optic cup and a ciliary margin, which are unique structures in the developing retina. In this review, we focus on self-organizing culture technique to generate 3D-retina from human ESCs.

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In Vivo Model of Small Intestine,ociated diseases. Herein, we provide a method for orthotropic transplantation of HIOs in immunocompromised mice. This method highlights the specific steps to successful engraftment and provides insight into the study of bioengineered human small intestine.

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1064-3745 ation advice from the experts.Includes supplementary materia.This volume provides readers with a better understanding of organogenesis in developmental biology and next-generation organ regenerative therapy. This book focuses on recent studies of organ regeneration from stem cells using in vitro 3D

燈絲

Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells, with correct structures by rearranging the source of the cells. In this article, we describe a detailed protocol for the regeneration of functional hair follicles and their stem cell niches by the rearrangement of embryonic or adult hair follicle-derived epithelial and mesenchymal cells.

過(guò)份

Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells,e kidney progenitor cells from pluripotent stem cells, which are based on embryological development. We also provide a method to generate 3D kidney tissue with vascularized glomeruli upon transplantation.

步兵

Generation of Various Telencephalic Regions from Human Embryonic Stem Cells in Three-Dimensional Cuegions along the dorsal-ventral (DV) axis by exogenous patterning signals. Here, we describe a protocol for in vitro generation of various telencephalic regions from human embryonic stem cells (ESCs). Dissociated human ESCs are reaggregated in a low-cell-adhesion 96-well plate and cultured as floati