Titlebook: Optical Tweezers; Methods and Protocol Arne Gennerich Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclus

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Art Ashkin and the Origins of Optical Trapping and Particle ManipulationA brief history of optical forces, the invention of optical tweezers, and their application to biological problems.

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978-1-0716-2231-5The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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Optical Tweezers978-1-0716-2229-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Synthesis of Germanium Nanospheres as High-Precision Optical Tweezers ProbesOPs), we could show that kinesin-1 walks with 4-nm-center-of-mass steps. In the long-term, the application of these novel high-precision GeNTOPs will provide new insight into the working mechanism of molecular machines and are promising candidates for other applications in microscopy, optoelectronics, and nanophotonics.

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Angular Optical Trapping to Directly Measure DNA Torsional Mechanics enables measurement of the twist persistence length of both extended DNA, under an extremely low force, and plectonemic DNA. This chapter can assist in the implementation and application of this technique for general researchers in the single-molecule field.

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CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Mic of safe and efficient therapeutics. Here, we describe methods for the use of optical tweezers combined with confocal fluorescence microscopy and microfluidics for the analysis of on- and off-target activity of Cas9 activity.

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1064-3745 expertsThis detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to st

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Book 2022Latest editionbroad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and mic