Titlebook: Optical Imaging of Neocortical Dynamics; Bruno Weber,Fritjof Helmchen Book 2014 Science+Business Media New York 2014 Cortical processing.F

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Optical Properties of Neural Tissueng properties of tissue influence imaging resolution, penetration depth, and often provide sources of contrast. Therefore, quantitative interpretation of imaging data requires knowledge of the optical properties of neural tissues. Light scattering in tissue arises from nanometer-scale spatial variat

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Fluorescent Indicators for Functional Optical Imagingring the last decade this approach was fueled by methodological progress in both optical instrumentation and fluorescent indicators of neuronal activities. In the first part of this chapter, we provide information required to reach an informed decision regarding the most suitable fluorescent indicat

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In Vivo Dendritic Mapping of Sensory Inputs in Cortical Neuronsn approach to identify sensory-evoked dendritic input sites in cortical neurons in vivo involving the use of two-photon calcium imaging combined with targeted whole-cell recordings. We provide basic technical descriptions as well as experimental procedures of this method. First, we discuss various s

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Chronic Two-Photon Imaging of Neural Activity in the Anesthetized and Awake Behaving Rodentpeatedly in different imaging sessions over months, opening new possibilities to investigate the function and plasticity of neuronal activity in both anesthetized and awake animals. A successful chronic imaging experiment involves the combination of several key techniques, including expression of ge

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