Titlebook: Opioid Receptors; Methods and Protocol Santi M. Spampinato Book 2015 Springer Science+Business Media New York 2015 Agonist ligands.Behavior

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Identification and Verification of Proteins Interacting with the Kappa Opioid Receptor (KOPR)motif-mediated ER retension of the hKOPR. Here we describe three experimental procedures we used to evaluate the interaction between hKOPR and 14-3-3zeta: co-immunoprecipitation, pull-down assay and immunofluorescence microscopy.

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Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissuesral sensory neuron cell bodies, along the axons and their peripheral endings in the hind paw skin, as well as in the spinal cord, under na?ve and sciatic nerve damage conditions in mice. Moreover, we consider the current debate on the specificity of antibodies.

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Fluorescence-Based, High-Throughput Assays for μ-Opioid Receptor Activation Using a Membrane Potentiane hyperpolarization, which is inhibited by opioids. Changes in membrane potential can be measured using a proprietary membrane potential-sensitive dye. In contrast to many HTS methods currently available, these assays reflect naturalistic coupling of the receptor to effector molecules.

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