Titlebook: Oligonucleotide-Based Therapies; Methods and Protocol Olof Gissberg,Rula Zain,Karin E. Lundin Book 2019 Springer Science+Business Media, LL

Corticosteroids

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Osmosis

Tissue-Specific Delivery of Oligonucleotidesl to the current CRISPR-Cas9 system offering gene editing at the genomic level, oligonucleotides, in addition to their biological functions in storing and conveying genetic information, provide the most prominent solutions to targeted gene therapies. Nonetheless, looking into the future of curing ca

CHECK

Designing siRNA and Evaluating Its Effect on RNA Targets Using qPCR and Western Blotunderstanding of gene function. Carefully designed siRNAs can considerably improve siRNA specificity leading to more accurate and efficient gene silencing. Evaluation of gene knockdown is vital for optimization of siRNA efficacy. Here we describe the fundamental principles of siRNA design and strate

貪婪地吃

Notorious

Assessing Oligonucleotide Binding to Double-Stranded DNAc approaches. Several methods can be used to evaluate ONs effect and binding capacity to their target sequence. Here we describe some of the methods, which have been frequently used for assessing ONs binding to dsDNA.

refraction

Modeling and Simulation of Oligonucleotide Hybrids: Outlining a Strategyres of biomolecules. Effects of chemical modifications and of the environment such as sequence, solvent, and ionic strength can explicitly be taken into account. Molecular simulation techniques can also provide insight in change in binding affinity, in protonation (p. shift) and tautomeric propensit

屈尊

600

Liability

Attachment of Peptides to Oligonucleotides on Solid Support Using Copper(I)-Catalyzed Huisgen 1,3-Di ONs with cell penetrating, homing or endosomal escape peptides can enhance specificity and/or uptake efficiencies. We describe here a general procedure for the preparation of peptide–oligonucleotide conjugates (POCs) on solid support utilizing a novel activated alkyne containing linker which enhanc

小平面

Characterizing Oligonucleotide Uptake in Cultured Cells: A Case Study Using AS1411 Aptamer of the target. The therapeutic potential of oligonucleotides targeted to intracellular molecules will depend largely on their ability to be taken up by the cells of interest, as well as their subsequent subcellular distribution. Here we describe methods to characterize the extent and mechanism of c