Titlebook: Olfactory Receptors; Methods and Protocol Fabio Marques Simoes de Souza,Gabriela Antunes Book 2018 Springer Science+Business Media, LLC, pa

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1064-3745 ns key implementation advice from the expertsThis detailed volume explores olfactory receptors, a topic that goes beyond classical biochemical essays and involves methods to investigate the brain areas associated with the perception of odorants. Gathering the strands of many advanced techniques, the

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Virus-Mediated Overexpression of Vomeronasal Receptors and Functional Assessment by Live-Cell Calcied VNO neurons and to characterize corresponding cell responses to potential ligands through Ca. imaging. This methodology enables us to analyze the response patterns of single vomeronasal receptors to a large number of chemosensory stimuli.

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Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations,he presence of different DNA epigenetic modification marks. Here we describe a method to separate a subpopulation of olfactory sensory neurons expressing the odorant receptor .. In this method, the main olfactory epithelium from transgenic .-IRES-GFP mice is dissociated into single cells, followed by separation of the GFP positive cells by FACS.

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Numerical Models and In Vitro Assays to Study Odorant Receptors, of already existing data through machine learning methods. This approach allows establishing predictions that map the chemical space and the receptor space. Then, we describe the method for assessing the activation of odorant receptors and their mutants through luciferase reporter gene functional assays.

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In Vivo Two-Photon Imaging of the Olfactory System in Insects,rain tissue labeling for in vivo imaging. Finally, we give an overview of the main methods of image data processing and analysis, followed by a short description of pioneering applications of this imaging modality.

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