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Titlebook: Nucleic Acids Hybridization; Modern Applications Anton A. Buzdin,Sergey A. Lukyanov Book 2007 Springer Science+Business Media B.V. 2007 DNA

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書目名稱Nucleic Acids Hybridization
副標(biāo)題Modern Applications
編輯Anton A. Buzdin,Sergey A. Lukyanov
視頻videohttp://file.papertrans.cn/669/668785/668785.mp4
概述In-depth review of nucleic acid hybridization in vitro techniques.All major applications covered.Includes novel methods with the potential of being widely used.Detailed experimental protocols provided
圖書封面Titlebook: Nucleic Acids Hybridization; Modern Applications Anton A. Buzdin,Sergey A. Lukyanov Book 2007 Springer Science+Business Media B.V. 2007 DNA
出版日期Book 2007
關(guān)鍵詞DNA; Microarray; Mutation; PCR; Promoter; evolution; hybridization; molecular biology
版次1
doihttps://doi.org/10.1007/978-1-4020-6040-3
isbn_softcover978-90-481-7512-3
isbn_ebook978-1-4020-6040-3
copyrightSpringer Science+Business Media B.V. 2007
The information of publication is updating

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Normalization of cDNA Libraries, genes transcribed at relatively low levels or for functional screenings. Here, we observed different cDNA libraries normalization methods, which were based on hybridization (renaturation) of cDNA or DNA, or RNA. Also we described duplex-specific nuclease (DSN) normalization protocol – simple and effective cDNA libraries normalization method.
地板
發(fā)表于 2025-3-22 05:20:59 | 只看該作者
Sergey A. Lukyanov,Konstantin A. Lukyanov,Nadezhda G. Gurskaya,Ekaterina A. Bogdanova,Anton A. Buzdi
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Nucleic Acids Hybridization: Potentials and Limitations,he large-scale recovery of DNA polymorphisms, including single nucleotide polymorphisms (SNPs). This book will focus on the above-mentioned and other recent developments in the area of nucleic acids hybridization, including attempts to improve its specificity. In this introductory chapter, I have tr
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Suppression Subtractive Hybridization,ary. SSH technique is applicable to many comparative and functional genetic studies for the identification of disease, developmental, tissuespecific, or other differentially expressed genes, as well as for the recovery of genomic DNA fragments distinguishing the samples under comparison. This chapte
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Primer Extension Enrichment Reaction (PEER) and Other Methods for Difference Screening, intended to replace the existing elegant hybridization methods but to expand their scope. PEER is tailored to find unknown targets present in very low copy numbers and in the context of an imperfect genomic match. The PEER method takes advantage of the greater hybridization specificity of shorter o
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Subtractive Hybridization with Covalently Modified Oligonucleotides, expressed genes in the tester can be preserved for RT–PCR amplification. To improve the efficiency of subtractive hybridization, we have developed a chemical modification procedure to generate covalently binding cDNAs as the subtracter to capture the homologous tester sequences. We have also proved
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