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Titlebook: Nucleic Acids; John M. Walker Book 1984 Humana Press 1984

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Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions,lecules is left intact during electrophoresis. The first method describes electrophoresis in a 2% (w/v) agarose gel in a dilute, neutral phosphate buffer. The second deals with electrophoresis in a linear gradient of polyacrylamide based on the buffer system of Loening (.).
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DNA Directed in Vitro Protein Synthesis with , S-30 Extracts,extract contains the machinery necessary for the transcription and translation, i.e., ribosomes and RNA polymerase. To this system, it is necessary to add all 20 amino acids, all four ribonucleotide triphosphates, transfer RNA, an energy generating system, and various salts. The DNA template is incu
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ctomized animals, and suggested that this was due to a hormone produced in the parathyroid gland. The story of parathyroid hormone discovery was indicative of ever-increasing sophistication in sample preparation and protein isolation techniques. This paper resolved earlier controversies over the fun
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