Titlebook: Nucleic Acid and Peptide Aptamers; Methods and Protocol Günter Mayer Book 2009 Humana Press 2009 Ligands.Protein modification.RNA.Target mo

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In Vitro Selection of ssDNA Aptamers Using Biotinylated Target ProteinsAptamers have been described for a series of target molecules including peptides, proteins, and even living cells. Besides RNA and 2′-modified RNA molecules also ssDNA molecules can be subjected to in vitro selection protocols aiming at the enrichment of ssDNA aptamers. ssDNA aptamers can be selecte

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Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interactionons based on a change of diffusion times (Rigler and Elson, (2001) .. Springer, Berlin; Haustein, and Schwille, (2004) .., 531–540). This technique can be applied to determine the dissociation constant of a complex formed by a fluorescence-labelled target and its corresponding RNA aptamer selected v

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Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallographyin RNA purification and crystallographic methods have lead to the visualization of an increasing number of unique structures, providing new insights into its function at the atomic level. This article presents general protocols which have streamlined the process of obtaining a homogeneous sample of

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