Titlebook: Nucleic Acid Crystallography; Methods and Protocol Eric Ennifar Book 2016 Springer Science+Business Media New York 2016 DNA.Macromolecule P

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Soaking Hexammine Cations into RNA Crystals to Obtain Derivatives for Phasing Diffraction Datals available for solving protein structures are not available for RNA. We have developed a reliable means to use hexammine cations to address this challenge. The process involves engineering the RNA to introduce a reliable hexammine binding site into the structure, then soaking crystals of these RNA

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Helical Symmetry of Nucleic Acids: Obstacle or Help in Structure Solution?ing continuous helices in the crystal. This arrangement of individual molecules in “infinite” pseudo helical structures in crystal may be the reason why the molecular replacement fails to find a unique position of the search atomic model as the method requires. The Patterson function, calculated as

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1064-3745 Protocols.?presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands..978-1-4939-4641-9978-1-4939-2763-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Preparation of Short 5′-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical nucleotide for structural and biochemical applications. The chapter describes the principles of construct design, in vitro transcription and RNA purification applied for characterization of a protein that targets the 5′ end of RNA.

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Preparation and Crystallization of Riboswitchesol, it is important to elucidate how riboswitches interact with cognate and discriminate against non-cognate ligands. Here we outline the methodology to synthesize riboswitch RNAs and prepare riboswitch–ligand complexes for crystallographic and biochemical studies. The chapter describes how to desig