Titlebook: Nucleic Acid Aptamers; Selection, Character Günter Mayer Book 2016 Springer Science+Business Media New York 2016 Aptamer generation process

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Capillary Electrophoresis for the Selection of DNA Aptamers Recognizing Activated Protein CCE-SELEX has a number of advantages over conventional SELEX procedures such as the selection of aptamers can be performed on non-immobilized targets, usually within a fewer number of selection cycles. Here we describe a complete procedure of CE-SELEX using activated protein C (APC) as the target pro

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Preparation of SELEX Samples for Next-Generation Sequencingce drops could be achieved, rendering next-generation sequencing very attractive for deep sequencing of SELEX libraries. Herein we describe the preparation of SELEX samples for Illumina sequencing, based on the already established whole genome sequencing workflow. We describe the addition of barcode

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Next-Generation Analysis of Deep Sequencing Data: Bringing Light into the Black Box of SELEX Experimsis of the SELEX procedure (Systematic Evolution of Ligands by EXponential enrichment) in an unprecedented depth and improves the identification of aptamers. Besides quality control and optimization of starting libraries, advanced screening strategies for difficult targets or early identification of

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Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothinding reaction and is therefore well suited for the analysis of small molecule—RNA aptamer interaction. We will introduce you to the method and present a protocol for sample preparation and the calorimetric measurement. A detailed note section will point out useful tips and pitfalls.

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Apta-PCRs method takes advantage of the combination of the sensitivity of nucleic acid amplification with the selectivity of aptamers. Ultra-low detection of target analyte can potentially be achieved, or, improved detection limits can be achieved with aptamers of low-medium affinity. Herein, we describe a