Titlebook: Nuclear Reprogramming; Methods and Protocol Nathalie Beaujean,Hélène Jammes,Alice Jouneau Book 2015Latest edition Springer Science+Business

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Visualization of Epigenetic Modifications in Preimplantation Embryos,lize histone modifications in living embryos, a Fab-based live endogenous modification labeling (FabLEM) technique has been developed. Here we describe the methods required for FabLEM experiments, including Fab preparation from IgG, its conjugation with a fluorescent dye, loading into cultured cells or mouse embryos, and imaging.

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隱語(yǔ)

978-1-4939-5441-4Springer Science+Business Media New York 2015

發(fā)芽

Nuclear Reprogramming978-1-4939-1594-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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流出

Nathalie Beaujean,Hélène Jammes,Alice JouneauIncludes fully updated, cutting-edge methods and protocols for nuclear reprogramming.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from t

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Nuclear Transfer in the Mouse,em (ntES) cells from living or frozen animals. The Piezo-driven direct microinjection NT method has proved to be a valid technique to clone mice and other species. In addition, this method has been broadly used as a versatile tool for many fields of mouse micromanipulation. This chapter describes th

Processes

Nuclear Transfer in Rabbit, of the mammalian species considered as difficult to clone. This chapter presents the technical protocol used nowadays to achieve the goal to obtain cloned embryos able to develop to term using fresh somatic cumulus cells.