Titlebook: Nuclear Reprogramming; Methods and Protocol Kejin Hu Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 iPSC repr

樂(lè)器演奏者

Cloning of Monkeys by Somatic Cell Nuclear Transfer,omedical research. We have recently cloned monkeys by optimization of the SCNT protocols and inclusion of the epigenetic modulator. Here, we describe the protocol for generation of cloned monkeys by somatic cell nuclear transfer.

JAUNT

Production of Cardiomyocyte-Like Cells by Fibroblast Reprogramming with Defined Factors,s into another type of differentiated cells, such as direct reprogramming of fibroblasts into cardiomyocytes, without passage through an undifferentiated pluripotent stage. Discovery of direct cardiac reprogramming offers a promising therapeutic strategy to prevent/attenuate cardiac fibrotic remodel

Initial

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Direct Reprogramming of Human Fibroblasts into Induced Neural Progenitor Cells Using Suicide Gene E This phenomenon can be mediated in the starting somatic cells by the introduction of lineage-specific master transcription factors or by pluripotency factors routinely used in iPS cell generation. In the latter process known as .luripotency factor-mediated .irect .eprogramming (PDR), the pluripoten

patriot

Generation of Human Neurons by microRNA-Mediated Direct Conversion of Dermal Fibroblasts,ing the fibroblast identity and evoking a pan-neuronal state. In contrast to induced pluripotent stem cell-derived neurons, miRNA-induced neurons (miNs) retain the biological age of the starting fibroblasts through direct fate conversion and thus provide a human neuron-based platform to study cellul

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Fillet,Filet

Panacea

Generation of Human iPSCs by Episomal Reprogramming of Skin Fibroblasts and Peripheral Blood MononuT4, SOX2, KLF4, and c-MYC. Sustained expression of the transgenes during reprogramming is crucial for the successful derivation of iPSCs. Integrating retroviruses have been used to achieve the required prolonged expression; however, issues of undesirable transgene expression in the iPSC-derived cell

ANTI

Generation of Human iPSCs by Protein Reprogramming and Stimulation of TLR3 Signaling,ells (e.g., fibroblasts), without the need for destruction of human embryos. This provides an unprecedented opportunity where patient-specific iPSCs can be subsequently differentiated to many cell types, e.g., cardiac cells and neurons, so that we can use these iPSC-derived cells to study patient-sp

prosthesis