Titlebook: Nuclear Reprogramming; Methods and Protocol Steve Pells Book 2006 Humana Press 2006 Activation.DNA.Ethylen.Monoclonal Antibodies.PCR.Telome

泰然自若

1064-3745 eosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.978-1-61737-530-9978-1-59745-005-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

shrill

Nuclear Transplantation in ,,ifferentiating cells, and larval-differentiated cells. Transplanted nuclei are reprogrammed to entirely new patterns of gene expression. In this chapter, the methods used to transplant living nuclei into enucleated eggs of . are described. A method also is described for transplanting multiple somati

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Protocols for Nuclear Transfer in Mice,tulate the developmental program. This chapter summarizes the method used since 1998 for mouse cloning, which differs from that for large animal cloning. A Piezo-drill micromanipulator allows direct injection of nuclei into enucleated mice oocytes instead of the electrofusion method used for introdu

OTTER

Isolation of Stromal Stem Cells From Human Adipose Tissue,l. Stromal stem cells offer a potentially large therapeutic potential in the field of regenerative medicine. Adipose tissue contains a large number of stromal stem cells, is relatively easy to obtain in large quantities, and thus constitutes a very convenient source of stromal stem cells. Importantl

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Nuclear Reprogramming by Cell Fusion,e expression was pioneered by Harris and Ringertz more than three decades ago. The ability to make heterokaryons with cells from different species or genetic strains is especially useful because genetic differences in gene products allow the origin of .-acting regulatory factors to be determined. He

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aplomb

Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells,cells between mouse embryonic stem cells and somatic cells, which is a type of nonchemically induced and nonvirus-mediated cell fusion, is introduced here as a highly effective, reproducible, and biomedically safe in vitro system. Under optimized electrofusion conditions, cells are aligned and form

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