Titlebook: Nuclear Bodies and Noncoding RNAs; Methods and Protocol Shinichi Nakagawa,Tetsuro Hirose Book 2015 Springer Science+Business Media New York

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Jeffrey J. Quinn,Howard Y. Chang must be examined closely if the contemporary politics of migration in Europe and the scope for their ‘Europeanization’ are to be understood. To do so we must go beyond the assumption that there is some simple linear process by which EU competencies are straightforwardly translated into domestic political change in the member states.

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Visualization of lncRNA by Single-Molecule Fluorescence In Situ Hybridizationification and spatial resolution of single RNA molecules within cells via hybridization of multiple, labeled nucleic acid probes to a target RNA. It has recently become apparent that single-molecule RNA FISH probes targeting noncoding RNA are more prone to off-target binding yielding spurious result

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Super-Resolution Imaging of Nuclear Bodies by STED Microscopyf light microscopy and thus requires electron microscopy for direct observation. Recent developments in super-resolution microscopy have extended the resolution of light microscopy to beyond 100 nm. Here, we describe a super-resolution technique, gated STED, for the analysis of the structure of nucl

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High-Resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrationse expression is regulated by distal regulatory sequences such as enhancers. Chromosome conformation capture (3C) techniques have recently revealed that the interactions between regulatory elements appear to occur in the context of topologically associating domains (TADs), each spanning few hundreds

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Visualization of Nucleic Acids with Synthetic Exciton-Controlled Fluorescent Oligonucleotide Probeshnologies. Herein, we describe a rapid and effective visualization method of nucleic acids in both fixed and living cells with hybridization-sensitive fluorescent oligonucleotide probes. These probes are efficiently quenched in an aqueous environment due to the homodimeric, excitonic interactions be

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Live CLEM Imaging to Analyze Nuclear Structures at High Resolutionrmation about cellular proteins and structures in living cells. EM provides nanometer resolution images of cellular structures in fixed cells. We have combined FM and EM to develop a new method of correlative light and electron microscopy (CLEM), called “Live CLEM.” In this method, the dynamic behav

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