Titlebook: Nitrogen Fixation; Methods and Protocol Markus W. Ribbe Book 2011 Springer Science+Business Media, LLC 2011 Biogenesis.Biosynthetic mechani

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Magnetic Circular Dichroism Spectroscopynts an effective way of obtaining structure/electronic information of paramagnetic metal ions. The basics of this technique are discussed along with examples of how MCD spectroscopy has been successfully used to elucidate the metal clusters of Nif proteins from nitrogen-fixing bacteria.

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1064-3745 -step methodologies.Includes key tips and expert implementat.Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-d

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Assays of Nitrogenase Reaction Productsof nitrogenase-catalyzed reactions involving nitrogen–nitrogen bonds, nitrogen–oxygen bonds, carbon–nitrogen bonds, carbon–carbon bonds, carbon–oxygen bonds, carbon–sulfur bonds, and hydrogen only. The usefulness of measurements of residual amounts of other components of nitrogenase assays is also discussed.

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X-Ray Absorption Spectroscopytype of ligands. Here, a basic introduction to XAS theory, the information that can be obtained, and the experimental consideration are presented. The application of XAS to biological systems and the impact this has had on nitrogenase research are briefly highlighted. New experimental advances are described.