Titlebook: Nitric Oxide; Methods and Protocol Alexander Mengel,Christian Lindermayr Book 2018 Springer Science+Business Media, LLC, part of Springer N

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Identification of S-Nitrosylated and Reversibly Oxidized Proteins by Fluorescence Switch and Complemnaling. The biotin switch technique for specifically labeling S-nitrosylated proteins opened the way to proteomic identification of these modifications. Since then, several variations and adaptations of the original method have been applied..We describe here the protocols of several techniques that

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Surface Plasmon Resonance Spectroscopy for Detection of ,-Nitrosylated Proteins The biotin-switch technique (BST) has become a mainstay method for detection of .nitrosylated proteins in biological samples. On the basis of BST, we describe a surface plasmon resonance (SPR) spectroscopy method for detecting .nitrosylated proteins. This method can be applied for the indirect dete

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Measurement of , -Nitrosoglutathione in Plasma by Liquid Chromatography–Tandem Mass Spectrometry.-nitrosoglutathione (GSNO) in human plasma. .-[.N]Nitrosoglutathione (GS.NO) serves as the internal standard. The protocol involves inactivation of plasma γ-glutamyltransferase activity by serine-borate, stabilization of GSNO with EDTA, and avoidance of .-transnitrosylation reactions by blocking SH

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Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Techniqueanslational modifications of proteins through its direct or indirect reaction with specific amino acid residues. Among them, S-nitrosylation has been shown to be involved in a broad range of cellular signaling pathways both in animals and plants. The identification of S-nitrosylated proteins has bee

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Identification of Tyrosine and Nitrotyrosine with a Mixed-Mode Solid-Phase Extraction Cleanup Followoteins. Different methods have been developed for identifying nitrotyrosine in biological samples. However, these analyses are difficult because tyrosine nitration is a very low-abundance posttranslational protein modification (PTM) and the lack of efficient enrichment methods for detection. The ide

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Electrophoretic Detection and Confocal Microscopic Imaging of Tyrosine Nitrated Proteins in Plant Tiscopy (CLSM) imaging. One-dimensional polyacrylamide gel electrophoresis (1D PAGE) followed by Western blotting using polyclonal antibody against 3-nitrotyrosine residues enables detection of tyrosine nitrated proteins in plant cells. Here we describe detection of tyrosine nitrated proteins in the h