Titlebook: Nicotinic Acetylcholine Receptor; Structure and Functi Alfred Maelicke Conference proceedings 1986 Springer-Verlag Berlin Heidelberg 1986 c

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Monoclonal Antibodies as Probes of Acetylcholine Receptor Functionodies have been developed (Watters and Maelicke, 1982, 1983; Fels et al., 1985, 1986). Here we summarize and discuss some recent data from our laboratory obtained with such antibodies. As the key results, (i) we have identified antibodies which affect receptor function indirectly, i.e. by allosteric

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Single Channel Studies of Acetylcholine Receptors Covalently Alkylated with Acetylcholineetween mean open time and agonist occupancy has not yet been tested quantitatively. (ii) Persistent depolarization after covalent labeling with agonist has only been observed in unclamped membrane voltage studies with . electroplaque cells (Bartels et al., 1976). Comparable studies with voltage-clam

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Effects of Allosteric Ligands on the Gating of Single Channel Currents in BC3H-1 Cells the open time distributions. In the presence of 100 nM ACh, tetracaine, PCP, and ANMC decreased apparent mean channel open times with IC. ’s of 2.5 ±.5 μM, 5.0 ±.5μM, and 3.0 ±.5μM, respectively. The effects of tetracaine were inconsistent with a model for sequential block of open channels. The num

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