Titlebook: Next Generation Sequencing and Data Analysis; Melanie Kappelmann-Fenzl Textbook 2021 Springer Nature Switzerland AG 2021 High-throughput-e

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Computer Setup,This chapter describes the minimum hardware and software requirements to analyze Next-Generation Sequencing data. There are various solutions to set up a compatible computer environment. Here, the basic installation steps are clearly presented and the fields of application of some important bioinformatic tools are described.

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Next Generation Sequencing (NGS): What Can Be Sequenced?,ain principle knowledge on DNA and RNA and understand the differences of analyzing these by sequencing templates. Next generation sequencing technologies can be used to sequence DNA or RNA, respectively, answering the research or clinical question you have. Different sequencing approaches thus lead to different readouts.

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NGS Technologies,iew of the most common technologies and their basic properties shall be given. Furthermore, it will be shown which technologies can be used for specific scientific or clinical questions and how they differ in their chemistry and output.

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Learning Materials in Bioscienceshttp://image.papertrans.cn/n/image/666227.jpg

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https://doi.org/10.1007/978-3-030-62490-3High-throughput-experiments; Next generation sequencing; genetic and epigenetic sequencing; NGS data-an

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NGS Data,ion sequencing data. Moreover, the structure and function of the different file formats are reviewed. This chapter explains how different file formats can be interpreted and what information can be gained from their analysis.