Titlebook: Next Generation Sequencing Technologies and Challenges in Sequence Assembly; Sara El-Metwally,Osama M. Ouda,Mohamed Helmy Book 2014 The Au

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Algorithms and Data Structures in Next-Generation SequencingThis chapter provides an overview of prevalent data structures and algorithms that are commonly utilized in bioinformatics. In particular, we place emphasis on data structures and algorithms that are employed in bioinformatic techniques during next-generation sequence assembly.

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First- and Next-Generations Sequencing Methodsa primary focus for biologists. Therefore, methodology for DNA sequencing has undergone rapid development, particularly since the 1970s. In this chapter, we will provide an overview of the majority of sequencing methods including techniques from the first- and next-generation of DNA sequencing.

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Next-Generation Sequencing Platformsng technologies available to individual laboratories. In this chapter, we will overview the major DNA sequencing platforms and present several comparisons for their advantages, disadvantages, sequencing costs, and other aspects.

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New Horizons in Next-Generation Sequencingthodologies and sequencers with outstanding features have also been released in the last few years. Furthermore, additional technologies demonstrating great promise are currently in development. In this chapter, we will briefly describe these recent and ongoing developments that may have a profound impact on the future of sequencing.

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Novel Next-Generation Sequencing Applications molecule to a whole new multitude of applications. In this chapter, we describe select novel applications of next-generation sequencing in relation to large-scale sequencing-based projects, cell and cell compartments sequencing and disease-targeted sequencing.

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