Titlebook: Neuroproteomics; Ka Wan Li Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2019 rodent brain regions

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David C. Hondius,Jeroen J. M. Hoozemans,Annemieke J. M. Rozemuller,August B. Smit that 60 % of noninfectious diseases are caused by errors in splicing, making the study of pre-mRNA splicing a high priority from a health perspective. Pre-mRNA splicing is also complicated: the molecular machine that catalyzes the reaction, the spliceosome, is composed of five small nuclear RNAs, a

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Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue, the fresh brain provides clear advantages, such as being able to see structures in very great detail by differences in color and shade, it also has some disadvantages. This particularly becomes evident when processing multiple samples within a short time-window, e.g., after a physiological stimulus

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Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centriiton X-100 and sucrose density centrifugation. The protocol also includes purification of other synapse sub-domains such as synaptosome, synaptic plasma membrane (SPM), synaptic membrane raft, PSD lattice, . (nuclei and cell debris), . (crude mitochondria fraction), . (soluble fraction), and . (micr

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High-pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics,H reversed-phase fractionation with low-pH LC-MS analysis is the method of choice, because it provides the optimal separation efficiency and deep protein coverage. In this chapter, we illustrate the protocols of HPLC-based and the tip-based high-pH reversed-phase chromatographic fractionation of pep

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Enrichment of Low-Molecular-Weight Phosphorylated Biomolecules Using Phos-Tag Tip,d biomolecules by using a phosphate-binding zinc(II) complex of 1,3-bis(pyridin-2-ylmethylamino)propan-2-olate (Phos-tag). A 200-μL micropipette tip containing 10 μL of swollen agarose beads functionalized with Phos-tag moieties (Phos-tag Tip) was prepared. All steps in the phosphate-affinity separa

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,Integrated Immunoprecipitation: Blue Native Gel Electrophoresis—Mass Spectrometry for the Identific and cellular state. To understand their biology, there is a need to identify the constituents in these various protein complexes. Conventional immunoprecipitation combined with proteomics analysis is nowadays routinely used to identify multiple interactors of a bait protein; however, it is unable t