Titlebook: Neuroprotection; Method and Protocols Swapan K. Ray Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to S

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Isolation of Capillaries from Small Amounts of Mouse Brain Tissue, are essential for analyzing changes in protein and gene expression at the BBB under physiological and pathological conditions. The standard methods for isolating brain capillaries require the use of at least one or more mouse brains in order to obtain sufficient quantity and purity of brain capilla

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Quantification of Neuroinflammatory Markers in Blood, Cerebrospinal Fluid, and Resected Brain Sampl therapeutic strategies, and they?can serve as outcome parameters due to their crucial role in the regulation of immune and inflammatory responses. It is vital to keep track of the entire cytokine spectrum due to the complex interactions, pleiotropic effects, and redundancy in the cytokine network.

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Targeted Modification of Epigenetic Marks Using CRISPR/dCas9-SunTag-Based Modular Epigenetic Toolkindromic repeats/dead CRISPR-associated protein 9 (CRISPR/dCas9) systems enable precise target gene-specific gene modulation by attaching different “effector” domains to the dCas9 protein to activate or repress specific genes. CRISPR/dCas9-SunTag is an improved system version, allowing more efficient

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Elevated Plus Maze for Assessment of Anxiety and Memory in Rodents,em (CNS) disorders. Unconditioned aversive behavior for open and elevated areas is a measure of anxiety and can be assessed by the plus maze. Plus maze consists of perpendicularly arranged open arms and closed arms crossed in the middle with a central platform. Rodents are allowed to explore the maz

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