Titlebook: Neuronal Cell Death; Methods and Protocol Arezu Jahani-Asl Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licen

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Performing Single-Cell Clonal Analysis in the Mouse Brain Using Mosaic Analysis with Double Markersnsiderations for experimental design. This powerful system can be adapted to make fundamental neuroscience discoveries by targeting genetically defined cell types in the mouse brain with high spatiotemporal resolution.

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Analysis of mRNA Dynamics Using RNA Sequencing Data,y this protocol to in vivo RNA-seq data from individuals with Alzheimer’s disease and demonstrate how estimates of differential stability can be leveraged to infer the regulatory factors underlying them.

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Superresolution Imaging of Cytoskeletal Networks in Fixed Brain Tissue, structured illumination microscopy is one of the most compatible methods for analyzing thicker?native tissue samples. We have developed a methodology that allows maximal preservation and quantitative analyses of cytoskeletal networks in tissue sections from a rodent brain. This methodology includes

Biomarker

Three-Dimensional Atlas of the Human Amygdala Subnuclei Constructed Using Immunohistochemical and Ul segmentations of the amygdala subnuclei on MR images, verified with immunohistochemical data, provide a robust three-dimensional atlas of the human amygdala. The goal is to apply the atlas to in vivo MRI scans to examine basolateral amygdala macrostructural development attributed to social cogniti

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Using Single-Molecule Fluorescence Microscopy to Uncover Neuronal Vulnerability to Protein Damage,what we have learned from culturing somatic nonneuronal cells. This chapter describes a method to investigate the induction of HSP70 in primary hippocampal neurons using single-molecule fluorescence in situ hybridization (smFISH). Quantification of smFISH provides the means to analyze neuron-to-neur

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Autopsy Human Brain Dissection Protocol for Common Age-Related Neurodegenerative Disorders,general knowledge of neuroanatomy of the human central nervous system. Tissue processing, detailed histological techniques and complete diagnostic examination of the brain is beyond the scope of this chapter; however, a limited evaluation appropriate for the evaluation of neurodegenerative disease i

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In Vitro Brain Organoids and Computational Models to Study Cell Death in Brain Diseases,ing and enables the formation of organoids with uniform size distributions. Next, we will introduce a mathematical framework that predicts the organoid growth, cell death, and the therapeutic responses of the organoids to different therapeutic agents. Through systematic investigations, the computati