Titlebook: Neuronal Cell Death; Methods and Protocol Laura Lossi,Adalberto Merighi Book 2015 Springer Science+Business Media New York 2015 DNA synthes

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Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosisuires transcription and protein synthesis, the complete spectrum of genes involved in distinct temporal domains remained unknown until the advent of genomics. In the last 10 years, the genome sequences and the development of high-throughput genomic technologies, such as DNA microarrays, have offered

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In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Deathr to perform OGD, cell or tissue cultures, such as primary neurons or organotypic slices, and acutely prepared tissue slices are usually incubated in a glucose-free medium under a deoxygenated atmosphere, for example in a hypoxic chamber. Here, we describe the step-by-step procedure to expose cultur

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Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy cell apoptosis has potential clinical value in early disease detection, as well as evaluating disease progression and treatment efficacy. Here, we describe our novel imaging technology DARC (Detection of Apoptosing Retinal Cells), which can be used to visualize single retinal neurons undergoing apo

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