Titlebook: Neural Transplantation Methods; Stephen B. Dunnett,Alan A. Boulton,Glen B. Baker Book 2000 Humana Press 2000

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Neural Stem Cells for Transplantation). Similar cells may also be isolated from human primary fetal tissues. Ethical and practical constraints on the procurement of viable human tissue emphasizes the worth of NSCs in providing an plentiful alternative source of human neural cells.

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Intracerebral Gene Transfer Using Viral Vectorsof genes in transgenic animals carrying a transgene surrounded by . sites (.), or by the production of molecules capable of inhibiting specific mRNAs, called ribozymes (.). Thus, the use of viral vectors has great potential, not only for the study of gene therapy (GT), but also for the study of the molecular basis of certain brain functions.

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Immortalized Cellsansion, and transplantation of immortalized neural cells is receiving attention from both neuroscientists and clinicians. For neuroscientists, immortalized neural cells may provide the raw material to understand the cellular and molecular mechanisms that occur during CNS development. Moreover, from

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Labeling and Identifying Grafted Cells. Similarly, prelabeling of xenografts is not necessary if species-specific markers can be employed, and nonneural cells, such as fibroblasts, can also be recognized in CNS tissue. The decision to explicitly label or not label donor material depends on a range of factors, including the nature of the