Titlebook: Neural Crest Cells; Methods and Protocol Quenten Schwarz,Sophie Wiszniak Book 2019 Springer Science+Business Media, LLC, part of Springer N

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Ex Vivo Culture and Manipulation of Mouse Neural Crest Cells from Primary Embryonic Tissue Explants This culture method can also be adapted for other molecular analyses, including addition of small-molecule inhibitors and/or growth factors to the culture media, as well as culturing neural crest cells from knockout or genetically modified mice.

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Proteomics in Neural Crest Cell Research,s an underutilized tool to study neural crest cell biology. Here we present a quantitative proteomics protocol for deep analysis of both whole proteome and posttranslational modifications and a separate protocol for ultrasensitive proteomic profiling from submicrogram amounts of protein.

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Mouse Embryo Culture for the Study of Neural Crest Cells,hots of mammalian neural crest cell development. This chapter describes methods for isolating and culturing mouse embryos and their organs in vitro, outside the mother, to facilitate real-time imaging and functional analyses of the dynamics of neural crest cell development.