Titlebook: Nanoscale Imaging; Methods and Protocol Yuri L. Lyubchenko Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 org

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1064-3745 ation advice from the experts.This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules i

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Book 2018cessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and through, .Nanoscale Imaging: Methods and Protocols. is a valuable resource for anyone interested in learning more about this developing and expanding field..

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semiskilled

Supported Lipid Bilayers for Atomic Force Microscopy Studiesefect-free POPC and POPS SLBs that remain stable for at least 8?h. The application of such smooth and stable surfaces is illustrated by monitoring of the on-surface aggregation of amyloid proteins with the use of time-lapse AFM.

Nonconformist

Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assaysf the utility of our single-molecule approaches, we show how human Dicer and its cofactor TRBP orchestrate the biogenesis of microRNA in real time. These single-molecule pull-down and fluorescence assays provide sub-second time resolution and can be applied to various ribonucleoprotein complexes that are essential for cellular processes.

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AFM-Based Single-Molecule Force Spectroscopy of Proteinsng. In this chapter we describe the experimental procedures for conducting a SMFS-AFM experiment—including purification of protein samples, setup and calibration of the AFM instrumentation, and the thorough and unbiased analysis of resulting AFM data.

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High-Resolution AFM-Based Force Spectroscopyain high force precision at enhanced bandwidths. Both modification methods allow site-specific attachment of biomolecules onto the apex area of the tips for force spectroscopy. The chapter concludes with a comparative demonstration using the two cantilever modification methods to study a lipid-protein interaction.

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AFM-Based Characterization of Electrical Properties of Materialsce gradients are explained. The applications of these techniques are illustrated on metals, surfactant compounds, semiconductors, and different polymers. Practical recommendations on use of the AFM-based electrical methods and the related challenges are given in the last section.

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Quantifying Small Molecule Binding Interactions with DNA Nanostructuresle stranded DNA fragments, and arbitrary small molecules. The described methods include microscale thermophoresis, ligand completion assays, circular dichroism spectroscopy, and AutoDock simulations. The protocols use organophosphates and model chemical nerve agents as examples, but the methods described here are broadly applicable.

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