Titlebook: NADPH Oxidases; Methods and Protocol Ulla G. Knaus,Thomas L. Leto Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2

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書目名稱NADPH Oxidases影響因子(影響力)




書目名稱NADPH Oxidases影響因子(影響力)學科排名




書目名稱NADPH Oxidases網(wǎng)絡公開度




書目名稱NADPH Oxidases網(wǎng)絡公開度學科排名




書目名稱NADPH Oxidases被引頻次




書目名稱NADPH Oxidases被引頻次學科排名




書目名稱NADPH Oxidases年度引用




書目名稱NADPH Oxidases年度引用學科排名




書目名稱NADPH Oxidases讀者反饋




書目名稱NADPH Oxidases讀者反饋學科排名

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衣服

Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assaysof this family of enzymes. These model systems have been used to analyze the biosynthesis and functional domains of NOX enzyme components as well as their regulation and cellular activities. This chapter provides an overview of the basic principles and applications of heterologous whole cell assays in studying NOX NADPH oxidases.

Hypopnea

良心

HPLC-Based Monitoring of Oxidation of Hydroethidine for the Detection of NADPH Oxidase-Derived Superquired for specific detection of the superoxide radical anion. Here, we provide protocols for HPLC- and LC-MS-based quantitation of 2-hydroxyethidium, among other oxidation products. Also, a protocol for continuous sampling for real-time monitoring of superoxide production using rapid HPLC measurements of 2-hydroxyethidium is described.

思想靈活

NOX5 Cell-Free Assay for the High-Throughput Screening of Small Moleculeseen debated. Here we describe a NOX5 cell-free assay. We present a method to purify the membranous component of cells stably transduced with NOX5 and to measure O.. in a high-throughput format (96-w or 384-w plates). The experimental description allows high-throughput screening of small molecules with limited cost.

EXALT

Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidasesvation prefers NOXO1 and NOXA1, although Rac is also involved. NOX3 constitutively produces superoxide, which is enhanced by regulatory proteins such as p47., NOXO1, and p67.. Here we describe mechanisms for NOX activation with special attention to the soluble regulatory proteins.

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