Titlebook: Microscopic Imaging Through Turbid Media; Monte Carlo Modeling Min Gu,Xiaosong Gan,Xiaoyuan Deng Book 2015 Springer-Verlag Berlin Heidelber

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Multiphoton Fluorescence Imaging,ncy of optical gating methods under single-photon (1p)?fluorescence imaging. Another important development in fluorescence imaging is the two-photon (2p) scanning fluorescence microscopy because of its advantages over 1p scanning fluorescence optical microscopy (Denk et al. Science 248:73–76, 1990;

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Image Reconstruction,et al., IEEE J Quantum Electron 23:1798–1805, 1987). In order to overcome this problem, approaches based either on the reduction of the scattering effect, e.g., two-photon (2p) fluorescence microscopy (Denk et al., Science 248:73–76, 1990; Saloma et al., Phys Med Biol 43:1741–1759, 1998), or on the

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Polarization-Gating Mechanism,s experimentally presented for a reflection optical microscope in Sect.?.. With the use of the Monte?Carlo simulation model, the polarization-gating performance in reflection and transmission optical microscopes is investigated in Sects.?. and ., respectively. Finally, the effective point spread fun

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Fluorescence-Gating Mechanism, experimental investigation will be given in Chap.?. together with comparisons with two-photon (2p) and three-photon (3p) fluorescence microscopy. In Sect.?., we define resolution and signal level since these two parameters are critical for imaging through a thick turbid medium. In Sects.?. and ., w

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Multiphoton Fluorescence Imaging,p fluorescence microscopy offers access to ultraviolet (UV) excitation without using UV lasers, and reduces Rayleigh scattering appreciably, provided that the size of scatterers in tissue is smaller than the illumination wavelength. According to these properties of 2p excitation, it has been claimed

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Image Reconstruction,391–394, 1991), spatial gating (Fujimoto et al., Opt Lett 3:150–152, 1986), and angle gating methods (Gan et al., Microsc Microanal 3:495–503, 1997), etc., to reduce the number of scattered photons, have been introduced. However, both approaches encounter the same difficulty of retaining strong sign

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1618-7210 Microscopic imaging through a tissue-like turbid medium can provide higher resolution than transillumination imaging in which no objective is used. This book serves as a valuable reference for engineers and scientists working on microscopy of tissue turbid media..978-3-662-51753-6978-3-662-46397-0Series ISSN 1618-7210 Series E-ISSN 2197-5647