Titlebook: Microinjection and Transgenesis; Strategies and Proto Angel Cid-Arregui,Alejandro García-Carrancá Book 1998 Springer-Verlag Berlin Heidelbe

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A New Strategy for Studying Microtubule Transport and Assembly During Axon Growthrning the mechanisms by which this occurs. The earliest model held that preassembled microtubules are transported from the cell body of the neuron down the growing axon, while subsequent models held that new polymer is added at the distal region of the growing axon via local microtubule assembly. Si

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Mouse Male Germ Cells in Culture: Toward a New Approach in Transgenesis? to methods based on the use of fertilized zygotes, early embryos, or ES cells. These powerful techniques are time consuming and costly. Moreover, they are applicable only to a limited number of species. The search for alternative methods appears therefore as a worthwhile long term prospect. Since t

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A Modified Protocol for the Generation of Transgenic Mice and Freezing of Mouse Embryoscribed in Hogan et al. (1986). Therefore, here we will only mention steps which deviate from those widely described. We have, over the years, simplified several steps, and consider it worthwhile to document this procedure.

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The Use of Morula Aggregation to Generate Germline Chimeras from Genetically Modified Embryonic Stemindefinitely in culture (see for review Robertson 1987). When incorporated into preimplantation stage embryos, ES cells are able to form chimeras. Such embryos develop into adult mice in which ES cells contribute to all tissues, including the germline (reviewed by Robertson 1986). These unique chara

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Angel Cid-Arregui,Carla Santana,Miriam Guido,Nestor Morales-Peza,Maria Victoria Juarez,Roger Wepf,Altural activities, unplanned urbanization, and imprudent management. In addition to these, inevitable climate change may further increase pressure on the water resources. An assessment of the water sources by using the agent-based modeling is necessary in order to understand stress on the available w