Titlebook: Microinjection; Methods and Applicat David J. Carroll Book 2009 Humana Press 2009 Antibodies.Expression.Gene function.Nucleotide.Protein ex

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Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complemout the roles of individual proteins in the cell. For many non-genetic model organisms researchers have relied on the use of inhibitory reagents, such as antibodies that can be microinjected into cells. More recently, the advent of RNA-mediated interference (RNAi) has allowed scientists to knockdown

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Quantitative Microinjection of Mouse Oocytes and Eggs,y large mammalian oocyte or egg is easily manipulated and can be injected with impermeant reagents including a variety of signaling molecules and fluorescent probes. Techniques have been developed to inject picoliter quantities of solution into oocytes and eggs with precision and reliability. The me

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Microinjection of Follicle-Enclosed Mouse Oocytes,and signals from the oocyte regulate the somatic cells. Because isolation of the oocyte from the follicle disrupts these communication pathways, oocyte physiology is best studied within an intact follicle. Here we describe methods for quantitative microinjection of follicle-enclosed mouse oocytes, t