Titlebook: Microfluidic and Compartmentalized Platforms for Neurobiological Research; Emilia Biffi Book 2015 Springer Science+Business Media New York

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A Novel In Vitro Primary Culture Model of the Lower Motor Neuron–Neuromuscular Junction Circuittomical and cellular interactions of this circuit in compartmented microfluidic devices, such that the glial cells are located with motor neuron cell bodies in the cell body chamber and motor neuron axons extend to a distal chamber containing skeletal muscle cells whilst simultaneously allowing targeted intervention.

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The Fabrication of Microfluidic Platforms with Pneumatically/Hydraulically Controlled PDMS Valves aners to investigate neurobiological phenomena in ways previously unachievable using traditional cell biology techniques. Here we detail the fabrication of a microfluidic cell coculture platform that uses pneumatically or hydraulically controlled valves to reversibly separate cell populations. Using t

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A Reliable Reversible Bonding Method for Perfused Microfluidic Devicessurface properties of the material itself. Bulk properties include optical transparency, gas permeability, and ease of fabrication, to name a few. On the other hand, silanol groups (SiOH) present on the surface can be easily activated through air/oxygen plasma treatments, and used to permanently bon

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Asymmetric Genetic Manipulation and Patch Clamp Recording of Neurons in a Microfluidic Chippartmentalized cell culture systems offer a simple yet powerful solution for isolation of axons from dendrites and cell somas. This chapter describes how to manufacture and use a microfluidic chip with a modular design for highly defined isolation of axons, asymmetric genetic manipulation, and whole

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Development of a Compartmentalized Biochip for Axonal Isolation and Neuronal-Circuit Formation at thy with the development of microelectrode arrays. Most current techniques used to form a defined neuronal network are based on microcontact-printing, but the intercellular connections in the patterned low-density network are formed randomly, systematic study of a specific network is not possible. For

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Campenot Cultures and Microfluidics Provide Complementary Platforms for Spatial Study of Dorsal Root These pseudo-unipolar neurons extend a single axon that bifurcates to innervate the periphery and spinal cord, allowing sensory information from the environment to be transferred rapidly to the central nervous system. During development, these DRG neurons rely on peripheral target-derived neurotrop

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Development of Microfluidic Devices for the Manipulation of Neuronal Synapsesamong these extracellular factors is the presence and influence of neighboring cells. It is crucial, therefore, in studying development to be able to replicate in vitro these network-like conditions. This is especially true of neuroscience, tissue engineering, and clinical biology, where network for