Titlebook: Microcephaly; Methods and Protocol Timothy Gershon Book 2023 Springer Science+Business Media, LLC, part of Springer Nature 2023 apoptosis.n

FLIT

scRNA-seq for Microcephaly Research [III]: Computational Analysis of scRNA-seq Dataaneously. Processing the complex data generated by scRNA-seq requires specialized computational tools. This chapter focuses on the analytical aspect of scRNA-seq workflow, with a focus on resolving biological signals from large-scale scRNA-seq data produced by the Drop-Seq platform.

STAT

Book 2023ne expression. All of the methods covered in this book use the mouse as a model system, and include cellular, metabolic, transcriptomic studies of neural progenitors, MR imaging studies of brain growth in 3-dimensions and developmentally-adapted studies of behavior in neonatal mice. Written in the h

谷物

Processing Neonatal Mouse Brains for Immunohistochemical Analysis: As Required for Including Spindle to allow sections without cracks or other anatomic disruptions. Moreover, paraffin embedding, which maximized tissue preservation, can reduce antigenicity of proteins in the embedded tissues. We describe an optimized embedding technique and antigen recovery technique that allows successful sectioning and immunohistochemical staining.

射手座

情節(jié)劇

Book 2023ials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. . Cutting-edge and comprehensive,?.Microcephaly: Methods and Protocols.?is a valuable resource for all researchers who want to expand their knowledge and understanding of this important and developing field..

Immunoglobulin

Conventional and Spectral Karyotyping of Murine Cerebellar Granule Neuron Progenitorsal rearrangements. Protocols for preparing slides (metaphase spreads) from fixed cell suspension, DAPI-banding, and spectral karyotyping (SKY) are included. Pertinent aspects of image acquisition and analysis are detailed. These protocols can likely be adapted to other tissue types (murine or human).

入會

Mouse Brain MRI: Including In Vivo, Ex Vivo, and fcMRI for the Study of Microcephalysystem parameters will be shown for anatomical imaging of in vivo and ex vivo brain tissue. Lastly, the chapter will detail a protocol for fcMRI along with a discussion of considerations specific to functional imaging.

呼吸

Maintaining Cerebellar Granule Neuron Progenitors in Cell Cultureifferentiation that increases over time, approximating their typical developmental trajectory. CGPNs in culture thus provide an ideal system for studying neural progenitor biology with the range of manipulations and analyses that are possible in vitro.

Hormones

Automated Immunofluorescence Staining for Analysis of DNA Damage and Apoptosis in Brain Sectionshe fractions of cells undergoing apoptosis, or both. While DNA damage in neural progenitors can trigger apoptosis, inappropriate apoptosis may also result from other processes. Simultaneous analysis of DNA damage and apoptosis in mouse models of microcephaly can determine how genetic instability and cell death contribute to the observed phenotype.

LATER

Whole-Body Mouse Fluxomic Analysis to Detect Metabolic Disruptions Associated with Microcephaly: Usie cell. However, a frozen snapshot of the interconnected, complex network of reactions gives very limited information about this system. Flux analysis using stable isotope labels enables more robust metabolic studies that consider interrogate metabolite processing and changes in molecular concentrations over time.