Titlebook: Microbiome Analysis; Methods and Protocol Robert G. Beiko,Will Hsiao,John Parkinson Book 2018 Springer Science+Business Media, LLC, part of

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16S rRNA Gene Analysis with QIIME2,r other community diversity analyses. The process of going from raw gene sequences to taxonomic profiles or diversity measures involves a series of data transformations performed by numerous computational tools. This includes tools for sequence quality checking, denoising, taxonomic classification,

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Normalization of Microbiome Profiling Data,ate or inadmissible—and all are narrowly relevant to a specific analysis that depends on both the nature of the data and the question being asked. This chapter describes key definitions of . as they apply in metagenomics, mainly for taxonomic profiling data; while also demonstrating specific, reprod

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Predicting the Functional Potential of the Microbiome from Marker Genes Using PICRUSt,ing does not provide direct information about the functional genes that are present in the genomes of community members. However, by capitalizing on the rapid growth in the number of sequenced genomes, it is possible to . which functions are likely associated with a marker gene based on its sequence