Titlebook: Microbial Systems Biology; Methods and Protocol Ali Navid Book 2012 Springer Science+Business Media, LLC 2012 NanoSIMS technology.bioanalyt

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Genetic Manipulation of the Obligate Chemolithoautotrophic Bacterium ,entral metabolism deviates from that of model organisms and there is a much less extensive experimental basis for their gene annotation than for typical organoheterotrophs. For microbes with sequenced genomes but unconventional metabolism, the ability to create knockout mutations can be a powerful t

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Genome-Wide Mapping of the Binding Sites of Proteins That Interact with DNAacterium to respond to an environmental signal, or to progress through a developmental program. Thus, in many situations, it is of interest to identify all of the genes that are under the control of a particular regulatory protein. This chapter begins with a brief overview of some of the methods tha

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Microbial Proteomics Using Mass Spectrometryain the optimal amount of required information about the test subject. Fortunately, many of these steps are common to most test subjects, requiring only modifications to or, in some cases, substitution of some of the steps to ensure they are relevant to the desired objective of a study. This fortuna

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Mass Spectrometry-Based Microbial Metabolomicsmetabolome, which is comprised of hundreds of chemically diverse metabolites, is rather difficult to monitor. Mass spectrometry (MS)-based profiling methods are increasingly being utilized for this purpose. To this end, MS is often coupled to the separation techniques gas chromatography (GC), liquid

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