Titlebook: MicroRNA Detection and Target Identification; Methods and Protocol Tamas Dalmay Book 2023Latest edition The Editor(s) (if applicable) and T

Monocle

MicroRNA Identification, Target Prediction, and Validation for Crop Improvement,h, the isolation of miRNAs is a tedious and difficult task using in vitro methods. However, recent exploration into the remarkably highly conserved nature of nucleotide sequences of miRNAs assists in the identification of miRNAs in plant species through homologous approaches. Here, we describe the i

Limpid

MicroRNA Detection with CRISPR/Cas, and therapeutic purposes. Their small size and low abundance, however, derive challenges in engineering robust detection tools. To date, multiple detection assays have been developed to achieve highly specific recognition of trace amount of miRNA with state-of-the-art nucleic acid detection and sig

CRACY

一回合

手榴彈

耕種

Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification,we detail a protocol for digital, isothermal, and multiplex detection of microRNAs, which relies on a recently developed amplification method. Our approach uses programmable ultrasensitive molecular amplifiers (PUMAs) to reveal the presence of target microRNAs randomly isolated in picoliter-size mic

Fierce

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Synapse

一美元

Determining miRNA Expression Patterns in Xenopus,by using a complementary labelled probe. MicroRNAs are short noncoding RNAs of 20–25?nt in length mainly involved in posttranscriptional regulation of gene expression. In this chapter, we describe how to visualize miRNAs in . or . by WISH using two different approaches: LNA-WISH to visualize mature